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Use a comma . . .
1. between independent clauses linked by a coordinating conjunction:
I saw this desk at a garage sale, and I bought it.
2. to set off long, introductory prepositional phrases:
Before making any assessments of the patient's condition, try performing this test.
3. to set off introductory dependent clauses:
When I learned the price of the desk, I realized that I could not afford it.
4. to set off introductory participal phrases:
Suffering from disease, the people of Manchester were victims of the revolution.
5. to set off introductory infinitive phrases:
To appreciate Gasket's sympathy for the poor, you should read Mary Barton..